Anti-Mouse OVA257-264 (SIINFEKL) peptide bound to H-2Kb APC

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

References: Porgador A, Yewdell JW, Deng Y, Bennink JR, Germain RN.Localization, quantitation, and in situ detection of specific peptide-MHC class I complexes using a monoclonal antibody.Immunity. 1997 Jun;6(6):715-26. (25-D1.16, mAb development, PubMed)

Messaoudi I, LeMaoult J, Nikolic-Zugic J.The mode of ligand recognition by two peptide:MHC class I-specific monoclonal antibodies.J Immunol. 1999 Sep 15;163(6):3286-94.

Ackerman AL, Kyritsis C, Tampé R, Cresswell P.Access of soluble antigens to the endoplasmic reticulum can explain cross-presentation by dendritic cells.Nat Immunol. 2005 Jan;6(1):107-13.

Berwin B, Hart JP, Rice S, Gass C, Pizzo SV, Post SR, Nicchitta CV.Scavenger receptor-A mediates gp96/GRP94 and calreticulin internalization by antigen-presenting cells.EMBO J. 2003 Nov 17;22(22):6127-36.