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Mouse MCP-5 Instant ELISA

Also known as: CCL12, Monocyte chemotactic protein 5, SCYA12

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS6007INST*

Cat. No. Size
BMS6007INST 128 tests
Please inquire about custom configurations or bulk packaging.

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Data for Mouse MCP-5 Instant ELISA.

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  • Data for Mouse MCP-5 Instant ELISA.
Description

Description: The mouse MCP-5 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monocyte chemoattractant protein-5. The mouse MCP-5 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

The chemokines are a large family of cytokines that control the recruitment of leukocytes in immune and inflammatory responses. Monocyte chemo attractant protein 5 (MCP-5) is a CC chemokine, based on its biological and structural features. Murine MCP-5 is mapped to the CC chemokine cluster on mouse chromosome 11 and is most closely related to human MCP-1 in structure. The chemokine is strongly chemotactic for monocytes and peritoncal macrophages, only weakly active on eosinophils and inactive on neutrophils. MCP-5 induces a calcium flux in peripheral blood mononuclear cells. Constitutive expression of this CC chemokine has been detected predominantly in lymph nodes, and its expression was markedly induced in macrophages.
MCP-5 is a potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens.

Details
Reactivity Mouse
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, heparin)
Sensitivity 2.3 pg/mL
Standard Curve Range 15.6 - 1,000 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with Polyclonal Antibody to mouse MCP-5, Biotin-Conjugate (anti-mouse MCP-5 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminium pouch(es) with a mouse MCP-5 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

References: Sarafi,M.N.; Garcia-Zepeda,E.A.; MacLean,J.A.; Charo,I.F.; Luster,A.D.. Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1. J.Exp.Med. 1997;185:99-109. (Link)

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