Anti-Mouse Ly-6C PerCP-Cyanine5.5

Also known as: Ly6C

Clone: HK1.4

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 45-5932

Cat. No.	Size
45-5932-80	25 ug
45-5932-82	100 ug
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Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC (cat. 11-0041) (left) and Anti-Mouse CD8a...View More

Description

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

In vitro addition of HK1.4 antibody can increase proliferation and stimulate cytokine release.

Details

Host	Rat
Isotype	IgG2c, kappa
Reactivity	Mouse
Conjugate	PerCP-Cyanine5.5
Laser	Blue Laser
Excite	488 nm
Legal	FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
17-5932	Anti-Mouse Ly-6C APC	633 - 647 nm	660 nm	FC	RUO
12-5932	Anti-Mouse Ly-6C PE	488 - 561 nm		FC	RUO
25-5932	Anti-Mouse Ly-6C PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
53-5932	Anti-Mouse Ly-6C Alexa Fluor® 488	488 nm	519 nm	FC	RUO
48-5932	Anti-Mouse Ly-6C eFluor® 450	405 nm		FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
11-0041	Anti-Mouse CD4 FITC	488 nm	518 nm	FC	RUO
12-0081	Anti-Mouse CD8a PE	488 - 561 nm		FC	RUO
17-5931	Anti-Mouse Ly-6G (Gr-1) APC	633 - 647 nm	660 nm	FC	RUO

References

References: Pflugh DL, Maher SE, Bothwell AL. Ly-6 superfamily members Ly-6A/E, Ly-6C, and Ly-6I recognize two potential ligands expressed by B lymphocytes. J Immunol. 2002 Nov 1;169(9):5130-6. (HK1.4, FC PubMed)

Havran WL, Lancki DW, Moldwin RL, Dialynas DP, Fitch FW. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes. J Immunol. 1988 Feb 15;140(4):1034-42. (HK1.4, FC, FA PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
Ly-6C	Innate Immunity	T Cells	PerCP-Cyanine5.5