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Anti-Mouse Ly-6C eFluor® 450

Clone: HK1.4

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 48-5932

Cat. No. Size
48-5932-80 25 ug
48-5932-82 100 ug

Data for Anti-Mouse Ly-6C eFluor® 450.

Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (cat. 11-0081) and 0.125 ug of Anti-Mouse...View More

  • Data for Anti-Mouse Ly-6C eFluor® 450.

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.

Host Rat
Isotype IgG2c, kappa
Reactivity Mouse
Conjugate eFluor 450
Laser Violet Laser
Emit 445 nm
Excite 405 nm
Reported Applications Flow Cytometric Analysis
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-5932 Anti-Mouse Ly-6C PE 488 - 561 nm 578 nm FC RUO
17-5932 Anti-Mouse Ly-6C APC 633 - 647 nm 660 nm FC RUO
47-5932 Anti-Mouse Ly-6C APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
45-5932 Anti-Mouse Ly-6C PerCP-Cyanine5.5 488 nm 695 nm FC RUO
16-5932 Anti-Mouse Ly-6C Functional Grade Purified FC, FA RUO
25-5932 Anti-Mouse Ly-6C PE-Cyanine7 488 - 561 nm 775 nm FC RUO
53-5932 Anti-Mouse Ly-6C Alexa Fluor® 488 488 nm 519 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0081 Anti-Mouse CD8a FITC 488 nm 520 nm FC RUO
17-0041 Anti-Mouse CD4 APC 633 - 647 nm 660 nm FC RUO

References: Vogelzang A, Lozza L, Reece ST, Perdomo C, Zedler U, Hahnke K, Oberbeck-Mueller D, Dorhoi A, Kaufmann SH. Neonatal Fc Receptor Regulation of Lung Immunoglobulin and CD103+ Dendritic Cells Confers Transient Susceptibility to Tuberculosis. Infect Immun. 2016 Sep 19;84(10):2914-21 (HK1.4, FC, PubMed)

Parlet CP, Kavanaugh JS, Horswill AR, Schlueter AJ. Chronic ethanol feeding increases the severity of Staphylococcus aureus skin infections by altering local host defenses. J Leukoc Biol. 2015 Apr;97(4):769-78. (HK1.4, FC, PubMed)

Pflugh DL, Maher SE, Bothwell AL. Ly-6 superfamily members Ly-6A/E, Ly-6C, and Ly-6I recognize two potential ligands expressed by B lymphocytes. J Immunol. 2002 Nov 1;169(9):5130-6. (HK1.4, FC PubMed)

Havran WL, Lancki DW, Moldwin RL, Dialynas DP, Fitch FW. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes. J Immunol. 1988 Feb 15;140(4):1034-42. (HK1.4, FC, FA PubMed)

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