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Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436

Clone: D7

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 62-5981

Cat. No. Size
62-5981-80 25 ug
62-5981-82 100 ug

Data for Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436.

Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (cat. 12-1172) and 0.125 ug...View More

  • Data for Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436.
Description

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Mouse
Conjugate Super Bright 436
Laser Violet Laser
Emit 436 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
61-5981 Anti-Mouse Ly-6A/E (Sca-1) PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
45-5981 Anti-Mouse Ly-6A/E (Sca-1) PerCP-Cyanine5.5 488 nm 695 nm FC RUO
25-5981 Anti-Mouse Ly-6A/E (Sca-1) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
13-5981 Anti-Mouse Ly-6A/E (Sca-1) Biotin FC RUO
16-5981 Anti-Mouse Ly-6A/E (Sca-1) Functional Grade Purified (Discontinued) FC, FA RUO
14-5981 Anti-Mouse Ly-6A/E (Sca-1) Purified FC, IP, WB RUO
12-5981 Anti-Mouse Ly-6A/E (Sca-1) PE 488 - 561 nm 578 nm FC RUO
15-5981 Anti-Mouse Ly-6A/E (Sca-1) PE-Cyanine5 488 - 561 nm 667 nm FC RUO
11-5981 Anti-Mouse Ly-6A/E (Sca-1) FITC 488 nm 520 nm FC RUO
56-5981 Anti-Mouse Ly-6A/E (Sca-1) Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
17-5981 Anti-Mouse Ly-6A/E (Sca-1) APC 633 - 647 nm 660 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-4222 Flow Cytometry Staining Buffer FC GPR
62-4321 Rat IgG2a K Isotype Control Super Bright 436 405 nm 436 nm FC RUO
12-1172 Anti-Mouse CD117 (c-Kit) PE 488 - 561 nm 578 nm FC RUO
SB-4400 Super Bright Staining Buffer FC GPR
References

References: Ortega, G., P. E. Korty, et al. (1986). Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol 137(10): 3240-6.

Malek, T. R., G. Ortega, et al. (1986). Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies. J Exp Med 164(3): 709-22.