Anti-Mouse Ly-49E/F APC

Description: This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F. The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).

References: Gays F, Martin K, Kenefeck R, Aust JG, Brooks CG. Multiple cytokines regulate the NK gene complex-encoded receptor repertoire of mature NK cells and T cells. J Immunol. 2005 Sep 1;175(5):2938-47. (CM4, FC, PubMed)

Stevenaert F, Van Beneden K, De Creus A, Debacker V, Plum J, Leclercq G. Ly49E expression points toward overlapping, but distinct, natural killer (NK) cell differentiation kinetics and potential of fetal versus adult lymphoid progenitors. J Leukoc Biol. 2003 Jun;73(6):731-8.

Van Beneden K, De Creus A, Stevenaert F, Debacker V, Plum J, Leclercq G. Expression of inhibitory receptors Ly49E and CD94/NKG2 on fetal thymic and adult epidermal TCR V gamma 3 lymphocytes. J Immunol. 2002 Apr 1;168(7):3295-302.