Anti-Mouse Ly-49A/D Purified

Description: The eBio12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.

eBio12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.

References: Mason LH, Anderson SK, Yokoyama WM, Smith HR, Winkler-Pickett R, Ortaldo JR. The Ly-49D receptor activates murine natural killer cells.J Exp Med. 1996 Dec 1;184(6):2119-28. (PubMed)

Ortaldo, J.R., et al. 1998. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J. Immunol. 160: 1158 - 1165.

Takei, F., et al. 1997. The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol. Rev. 155: 67 – 77.

Ortaldo, J.R., et al. 1999. Ly-49 receptor expression and functional analysis in multiple mouse strains. J. Leukoc. Biol. 66: 512 - 520.

Nakamura MC, Hayashi S, Niemi EC, Ryan JC, Seaman WE. Activating Ly-49D and inhibitory Ly-49A natural killer cell receptors demonstrate distinct requirements for interaction with H2-D(d). J Exp Med. 2000 Aug 7;192(3):447-54. (PubMed)