Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Description: Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated.
Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
Applications Reported: The intended use of the Recombinant Mouse IL-9 Single-Use ELISA RSG Standard is as a standard for mouse IL-9 Tissue culture Ready-SET-Go! ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below. Instructions for use: For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 8 µL of standard solution to 2 mL of assay diluent to prepare the top standard solution (4000 pg/mL).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.8 µL in 0.2 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the Mouse IL-9 Tissue Culture Ready-SET-Go! ELISA Set (Cat. No. 88-6952).
Source: Insect cells infected with baculovirus.
Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytokine P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; T-cell growth factor P40
Gene Aliases: Il9
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support