Mouse IL-5 ELISPOT Ready-SET-Go!®

Also known as: Interleukin-5, IL

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7825

Cat. No. Size
88-7825-21 2 x 96 tests
88-7825-88 10 x 96 tests
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Data for Mouse IL-5 ELISPOT Ready-SET-Go!®.

SJL splenocytes were stimulated with 2 µg/ml of ConA in HL-1 media for 48 hours (right well) in a...View More

  • Data for Mouse IL-5 ELISPOT Ready-SET-Go!®.
Description

Description: This Mouse IL-5 ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of IL-5-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.

Details
Reactivity Mouse
Components Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISPOT Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 uM).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
Reported Applications ELISPOT
Documentation
TDS Link Download TDS
References