Mouse IL-4 ELISPOT Ready-SET-Go!®

Also known as: Interleukin-4, IL4

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7844

Cat. No.	Size
88-7844-21	2 x 96 tests
88-7844-88	10 x 96 tests
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Splenocytes from 8-week old mice pre-immunized with OVA/IFA were again activated with OVA antigen...View More

Description

Description: This Mouse IL-4 ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of IL-4-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.

Details

Reactivity	Mouse
Components	Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISPOT Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 uM). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
Reported Applications	ELISPOT

Documentation

TDS Link	Download TDS

References

References: Anis MM, Fulton SA, et al. 2007. Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection. Infect Immun. 75(5):2260-8. (ELISPOT kit, PubMed)

View More Products

Antigens	Area of Biology	Cell Type
IL-4	Adaptive Immunity Cytokines, Chemokines, Growth Factors Innate Immunity	T Cells Th2 Cells