Anti-Mouse IL-27 p28 Functional Grade Purified

Description: This MM27-7B1 monoclonal antibody reacts with the p28 subunit of Interleukin-27 (IL-27) and neutralizes the bioactivity of IL-27, a member of the IL-12 family. IL-27 is a heterodimer of the subunits EBI3 (Epstein-Barr Virus Induced Gene 3), which is homologous to the p40 subunit shared by IL-12 and IL-23, and p28 (IL-30), which is homologous to p35. It is produced by activated dendritic cells and macrophages in response to TLR ligands and inflammatory cytokines.

The IL-27 receptor shares one subunit, gp130, with other members of the IL-6 family. The subunit WSX-1 (IL-27R alpha, TCCR) is unique to IL-27 and is believed to be the only part of the receptor that interacts with the cytokine. The IL-27R is most abundantly expressed on activated T-cells and NK cells, although expression has also been shown on B-cells and naïve T-cells. IL-27R activation leads to the phosphorylation of Jak/STAT proteins, with STAT1 and STAT3 being critical to the function of IL-27. IL-27 has been shown to have both pro-inflammatory and anti-inflammatory effects. It influences the commitment of CD4+ T-cells toward the Th1 lineage by inducing the expression of the T-bet transcription factor and the upregulation of IL-12R beta2. Its anti-inflammatory functions include the suppression of Th2 and Th17 proliferation and differentiation. Susceptibility to T-cell mediated autoimmunity has been observed in WSX-1 knockout mice.

Recent evidence suggests that the p28 subunit may also be secreted independently of EBI3 and have functions distinct to the IL-27 heterodimer. It is believed to not only antagonize the activity of IL-27, but also inhibit signaling of other gp130 ligands, such as IL-6 and IL-11.

References: Stumhofer JS, Tait ED, Quinn WJ 3rd, Hosken N, Spudy B, Goenka R, Fielding CA, O’Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA. A Role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat Immunol. 2010 Dec;11(12):1119-26.

Stumhofer JS and Hunter CA. Advances in understanding the anti-inflammatory properties of IL-27. Immunol Lett. 2008 May 15; 117(2): 123-30.

Yoshimura A, Yoshida H, Miyazaki Y, Kinjyo I, Ishibashi T, Yoshimura T, Takeda A, Hamano S. Two sided roles of IL- 27: induction of Th1 differentiation on Naïve CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol. 2006; 177: 5377-85.