Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

Description: This Mouse IL-23 Ready-SET-Go! ELISA Kit (including pre-coated ELISA plates) contains the necessary reagents, buffers and diluents for performing quantitative, enzyme-linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of mouse IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-23 proteins with a standard curve range of 30 pg/ml to 4,000 pg/ml and assay sensitivity below 30 pg/ml. Native mouse IL-23 was detected in supernatants from bone marrow-derived, LPS-activated dendritic cells. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this assay exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed; all values were at the limit of detection of the assay.

For measurement of IL-12 total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! is available (88-7120).

References: Happel, K.I., et al. 2005. Divergent roles of IL-23 and IL-12 in host defense against Klebsiella pneumoniae. J. Exp. Med. 202: 761 - 769. [Mouse IL-23 ELISA]
Kidoya, H., et al. 2005. Fas ligand induces cell-autonomous IL-23 production in dendritic cells, a mechanism for fas ligand-induced IL-17 production. J. Immunol. 275: 8024-8031. [Mouse IL-23 ELISA]
Zakharova, M. et al. 2005. Paradoxical Anti-Inflammatory Actions of TNF- : Inhibition of IL-12 and IL-23 via TNF Receptor 1 in Macrophages and Dendritic CellsJ. Immunol. 175: 5024 - 5033. [Mouse IL-23 ELISA]
Fichtner-Feigl, S., et al. 2005. Treatment of murine Th1- and Th2-mediated inflammatory bowel disease with NF-B decoy oligonucleotides. J. Clin. Invest. 115: 3057 - 3071. [Mouse IL-23 ELISA]
Kuwashima, N., et al. 2005. Delivery of Dendritic Cells Engineered to Secrete IFN- into Central Nervous System Tumors Enhances the Efficacy of Peripheral Tumor Cell Vaccines: Dependence on Apoptotic Pathways. J. Immunol. 175: 2730 - 2740. [Mouse IL-23 ELISA]
Hegazi, R.A.F., et al. 2005. Carbon monoxide ameliorates chronic murine colitis through a heme oxygenase 1–dependent pathway. J. Exp. Med. 202: 1703 - 1713. [Mouse IL-23 ELISA]