Description: This FlowCytomix Simplex Kit is designed for the measurement of Mouse IL-22 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Mouse Basic Kit (cat. BMS8440FF), this kit can be used to detect IL-22 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population A5.
IL-22/TIF(IL-10-related T cell-derived inducible factor) is a new cytokine originally identified as a gene induced by IL-9 in murine T lymphocytes, and showing 22% amino acid identity with IL-10. In the mouse, the IL-22 gene is located on chromosome 10, in the same region as the IFN gamma gene. Although it is a single copy gene in BALB/c and DBA/2 mice, the IL-22 gene is duplicated in other strains such as C57Bl/6, FVB and 129. The two copies, which show 98% nucleotide identity in the coding region, were named IL-TIF alpha and IL-TIF beta with the IL-TIF beta gene being either differentially regulated, or not expressed at all.
IL-22 is a cytokine originally identified as a gene induced by IL-9 in murine T lymphocytes, and showing 22% amino acid identity with IL-10.
IL-22 is produced by activated Th1 and NK cells acting primarily on epithelial cells and is involved in inflammatory responses. Neither resting nor activated immune cells express IL-22 receptor, and IL-22 does not have any effects on these cells in vitro and in vivo. In contrast, cells of the skin and the digestive and respiratory systems represent putative targets of this cytokine. Thus IL-22 does not serve the communication between immune cells but is a T cell mediator that directly promotes the innate, nonspecific immunity of tissues. IL-22 serves as a protective molecule to counteract the destructive nature of the immune response to limit tissue damage.