Mouse IL-2 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Mouse IL-2 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Mouse Basic Kit (cat. BMS8440FF), this kit can be used to detect IL-2 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A6.

Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response.
IL-2 gene expression is regulated at the transcriptional level by several activation pathways. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors for IL-2 induced in an autocrine fashion on the surface of T-cells.

Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon-gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T-cells to synthesize IL-2 have been observed in physiologic and pathologic states.

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Hildner,K.M.; Schirmacher,P.; Atreya,I.; Dittmayer,M.; Bartsch,B.; Galle,P.R.; Wirtz,S.; Neurath,M.F.. Targeting of the transcription factor STAT4 by antisense phosphorothioate oligonucleotides suppresses collagen-induced arthritis. J Immunol 2007;178:3427-3436. (Link)

Fletcher,Julie M.; Jordan,Margaret A.; Snelgrove,Sarah L.; Slattery,Robyn M.; Dufour,Francois D.; Kyparissoudis,Konstantinos; Besra,Gurdyal S.; Godfrey,Dale I.; Baxter,Alan G.. Congenic Analysis of the NKT Cell Control Gene Nkt2 Implicates the Peroxisomal Protein Pxmp4. The Journal of Immunology 2008;181:3400-3412. (Link)

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Mrabet-Dahbi,S.; Metz,M.; Dudeck,A.; Zuberbier,T.; Maurer,M.. Murine mast cells secrete a unique profile of cytokines and prostaglandins in response to distinct TLR2 ligands. Exp.Dermatol. 2009;18:437-444. (Link)

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Weigmann,Benno; Lehr,Hans A.; Yancopoulos,George; Valenzuela,David; Murphy,Andrew; Stevens,Sean; Schmidt,Jan; Galle,Peter R.; Rose-John,Stefan; Neurath,Markus F.. The transcription factor NFATc2 controls IL-6-dependent T cell activation in experimental colitis. Journal of Experimental Medicine 2008;205:2099-2110. (Link)

Fantini,M.C.; Becker,C.; Tubbe,I.; Nikolaev,A.; Lehr,H.A.; Galle,P.; Neurath,M.F.. Transforming growth factor {beta} induced FoxP3+ regulatory T cells suppress Th1 mediated experimental colitis. Gut 2006;55:671-680. (Link)

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