Mouse IL-1 beta ELISA Ready-SET-Go!®

Also known as: Interleukin-1 beta

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7013

Cat. No. Size
88-7013-22 2 x 96 tests w/plates
88-7013-76 20 x 96 tests w/plates
88-7013-77 20 x 96 tests
88-7013-86 10 x 96 tests w/plates
88-7013-88 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

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Data for Mouse IL-1 beta ELISA Ready-SET-Go!®.

Standard curve of Mouse IL-1 beta ELISA Ready-SET-Go!®...View More

  • Data for Mouse IL-1 beta ELISA Ready-SET-Go!®.
Description

Description: This Mouse IL-1 beta ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-1 beta protein levels from samples including serum, protein lysate and supernatants from cell cultures.

Special Note: To ensure optimal results from the Mouse IL-1 beta ELISA Ready-SET-Go! Set, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

Details
Reactivity Mouse
Sensitivity 8 pg/mL
Standard Curve Range 8 - 1000 pg/mL
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation
TDS Link Download TDS
References

References: Chen L, Lin S, et al. 2006. CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model. Int Immunol 18(8):1233-42. (RSG ELISA kit, protein lysate, PubMed)

Lu J, Sadri N, and Schneider RJ. 2006. Endotoxic shock in AUF1 knockout mice mediated by failure to degrade proinflammatory cytokine mRNAs. Genes Dev 20(22):3174-84. (RSG ELISA kit, TC supernatant, PubMed)

Cho HJ, Seon MR, et al. 2008. 3,3'-Diindolylmethane suppresses the inflammatory response to lipopolysaccharide in murine macrophages. J Nutr. 138(1):17-23. (RSG ELISA kit, TC supernatant, PubMed)