Mouse IL-17F (homodimer) ELISA Ready-SET-Go!® Set

Also known as: Interleukin-17F, IL17F, IL-17FF

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7472

Cat. No. Size
88-7472-22 2 x 96 tests w/plates
88-7472-88 10 x 96 tests
88-7472-86 10 x 96 tests w/plates

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Data for Mouse IL-17F (homodimer) ELISA Ready-SET-Go!® Set.

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Description

Description: This mouse IL-17F ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-17FF homodimer protein levels from samples including serum, plasma and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-17F proteins and a standard curve range of 15 to 2000 pg/ml. No crossreactivity to the IL-17AA homodimer is observed and <2.5% crossreactivity to IL-17AF heterodimer when spiked in excess (100ng/ml).

IL-17F is a member of the IL-17 family and a signature Th17 cytokine. Of the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity) and the two genes are located in the same chromosomal region. Although both IL-17A and IL-17F were originally found to be produced as disulfide-linked homodimers, recent studies have confirmed the production of IL-17A/IL-17F heterodimers in in vitro-differentiated and polarized Th17 cells. IL-17F and IL-17A have been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. IL-17F treatment of airway epithelium, vein endothelial cells, and fibroblasts has been reported to induce expression of IL-6, IL-8, GRO-a, ENA-78, TGF-ß, MCP-1, G-CSF, GM-CSF, and ICAM-1.

Details
Reactivity Mouse
Sensitivity 15 pg/ml
Standard Curve Range 15-2000 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Chang SH, Dong C. A novel heterodimeric cytokine consisting of IL-17 and IL-17F regulates inflammatory responses. Cell Res. 2007 May;17(5):435-40.

Wright JF, Guo Y, Quazi A, Luxenberg DP, Bennett F, Ross JF, Qiu Y, Whitters MJ, Tomkinson KN, Dunussi-Joannopoulos K, Carreno BM, Collins M, Wolfman NM. Identification of an interleukin 17F/17A heterodimer in activated human CD4+ T cells. J Biol Chem. 2007 May 4;282(18):13447-55. Epub 2007 Mar 13.

Liang SC, Tan XY, Luxenberg DP, Karim R, Dunussi-Joannopoulos K, Collins M, Fouser LA. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides. J Exp Med. 2006 Oct 2;203(10):2271-9. Epub 2006 Sep 18.