Mouse IL-17A (homodimer) ELISA Ready-SET-Go!®

Also known as: Interleukin-17A, IL-17AA

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7371

Cat. No. Size
88-7371-22 2 x 96 tests w/plates
88-7371-76 20 x 96 tests w/plates
88-7371-77 20 x 96 tests
88-7371-86 10 x 96 tests w/plates
88-7371-88 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

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Data for Mouse IL-17A (homodimer) ELISA Ready-SET-Go!®.

Standard curve of Mouse IL-17A (homodimer) ELISA Ready-SET-Go!®...View More

  • Data for Mouse IL-17A (homodimer) ELISA Ready-SET-Go!®.
Description

Description: This Mouse IL-17A ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-17AA homodimer protein levels from samples including serum, and supernatants from cell cultures.

Special Note: To ensure optimal results from the Mouse IL-17A ELISA Ready-SET-Go! Set, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

Details
Reactivity Mouse
Sensitivity 4 pg/mL
Standard Curve Range 4 - 500 pg/mL
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

References: Jarry A, Bossard C, Bou-Hanna C, Masson D, Espaze E, Denis MG, Laboisse CL. Mucosal IL-10 and TGF-beta play crucial roles in preventing LPS-driven, IFN-gamma-mediated epithelial damage in human colon explants. J Clin Invest. 2008 Mar;118(3):1132-42. (RSG ELISA kit, TC supernatant, PubMed)

Yoshimura T, Sonoda KH, Miyazaki Y, Iwakura Y, Ishibashi T, Yoshimura A, Yoshida H. Differential roles for IFN-gamma and IL-17 in experimental autoimmune uveoretinitis. Int Immunol. 2008 Feb;20(2):209-14. (RSG ELISA kit, TC supernatant, PubMed)

Xin L, Li Y, Soong L. Role of interleukin-1beta in activating the CD11c(high) CD45RB- dendritic cell subset and priming Leishmania amazonensis-specific CD4+ T cells in vitro and in vivo. Infect Immun. 2007 Oct;75(10):5018-26. (RSG ELISA kit, TC supernatant, PubMed)

Nishihara M, Ogura H, Ueda N, Tsuruoka M, Kitabayashi C, Tsuji F, Aono H, Ishihara K, Huseby E, Betz UA, Murakami M, Hirano T. IL-6-gp130-STAT3 in T cells directs the development of IL-17+ Th with a minimum effect on that of Treg in the steady state. Int Immunol. 2007 Jun;19(6):695-702. (RSG ELISA kit, serum, PubMed)