Description: This FlowCytomix Simplex Kit is designed for the measurement of Mouse IL-15R/IL-15 complex in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Mouse Basic Kit (cat. BMS8440FF), this kit can be used to detect the IL-15 complexed to IL-15 receptor alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population A11.
IL-15 is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK
cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling
through a heterotrimeric receptor. Two of the subunits of this receptor, beta
and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to
IL-15.
Despite the expression of IL-15 mRNA in many cell types, the secreted
protein is rarely detectable in biological samples. Recent research
suggests that IL-15 is retained inside the cell and is only secreted in
complex with its unique receptor, IL-15Ralpha. This chaperoning is required
from the ER to Golgi through to secretion. The cytokine/receptor
complex may stay bound to the cell surface, where it can be trans-
presented to cells expressing the beta-gamma subunits of the receptor, or may be
secreted in a soluble form that lacks the transmembrane domain of the
receptor. The soluble IL-15/IL-15R complex is produced by mouse
dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-
100-fold increase in agonistic activity over IL-15 alone.
