Mouse IL-15/IL-15R Complex Recombinant Protein Carrier-Free

Description: This recombinant protein is the heterocomplex of the mouse IL-15 linked to soluble IL-15 receptor alpha chain (sushi domain) via a GS linker.

Interleukin 15 (IL-15) is a cytokine that stimulates T and NK cell proliferation and differentiation. IL-15 regulates macrophage proinflammatory and chemotactic protein production. In addition, it induces differentiation of monocytes towards dendritic cells. The receptor for IL-15 is a high affinity heterotrimeric receptor composed of IL-15Rα and 2 additional subunits of the IL-2 receptor (beta and gamma). Upon activation, the receptor complex signals via the Stat and JAK pathways.

It has been reported that the soluble form of IL-15Rα corresponding to the entire extracellular domain, cleaved by MMP’s, behaves as a high affinity IL-15 antagonist, whereas the recombinant extracellular domain comprised of the sushi domain together with the ligand, IL-15, behaves as a potent agonist by enhancing binding of IL-15 to the receptor complex. In fact, this receptor-ligand complex has approximately 10-100 fold increased proliferative activity.

References: Mortier E, Quéméner A, Vusio P, Lorenzen I, Boublik Y, Grötzinger J, Plet A, Jacques Y. Soluble interleukin-15 receptor alpha (IL-15R alpha)-sushi as a selective and potent agonist of IL-15 action through IL-15R beta/gamma. Hyperagonist IL-15 x IL-15R alpha fusion proteins. J Biol Chem. 2006 Jan 20;281(3):1612-9.

Teague, R. M. Sather, B. D. Sacks, J. A. Huang, M. Z. Dossett, M. L. Morimoto, J. Tan, X. Sutton, S. E. Cooke, M. P. Ohlen, C. Greenberg, P. D. Interleukin-15 rescues tolerant CD8+ T cells for use in adoptive immunotherapy of established tumors. Nature Med. 2006; 12: 335-341.

Vamosi G, Bodnar A, Vereb G, Jenei A, Goldman CK, Langowski J, Toth K, Matyus L, Szollosi J, Waldmann TA, Damjanovich S. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11082-7.

Dubois S, Mariner J, Waldmann TA, Tagaya Y. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity. 2002 Nov;17(5):537-47.