Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Description: Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.
Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
Applications Reported: Mouse IL-15/IL-15R Complex Single-Use ELISA RSG Standard has been reported for use in ELISA.
Applications Tested: The intended use of the Mouse IL-15/IL-15R Complex Single-Use ELISA RSG Standard is as a standard for Mouse IL-15/IL-15R Complex ELISA (cat. 88-7215). Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below.
Instructions for use: Add 5 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (500 pg/mL).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 µL in 1.0 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD215; IL-15; IL-15 receptor subunit alpha; IL-15R-alpha; ILN; Interleukin; Interleukin-15; Interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; Soluble interleukin-15 receptor subunit alpha
Gene Aliases: Il15; Il15ra
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