Mouse IL-12 p70 ELISA Ready-SET-Go!®

Also known as: Interleukin-12, IL12, IL23

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7121

Cat. No. Size
88-7121-22 2 x 96 tests w/plates
88-7121-76 20 x 96 tests w/plates
88-7121-77 20 x 96 tests
88-7121-86 10 x 96 tests w/plates
88-7121-88 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

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Data for Mouse IL-12 p70 ELISA Ready-SET-Go!®.

Standard curve of Mouse IL-12 p70 ELISA Ready-SET-Go!® ...View More

  • Data for Mouse IL-12 p70 ELISA Ready-SET-Go!®.
Description

Description: This Mouse IL-12 p70 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This Mouse IL-12 p70 ELISA Ready-SET-Go! reagent set specifically measures the bioactive, heterodimeric form of IL-12, p70, without interference by p40 monomer or the related protein IL-23 (p19/p40). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-12 p70 protein levels from samples including serum, and supernatants from cell cultures.

Details
Reactivity Mouse
Sensitivity 15 pg/mL
Standard Curve Range 15 - 2000 pg/mL
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
14-8231 Mouse IL-23 Recombinant Protein ELISA, FA RUO
34-8231 Mouse IL-23 Recombinant Protein Carrier-Free FA RUO
References

References: Hsu SC, Tsai TH, Kawasaki H, Chen CH, Plunkett B, Lee RT, Lee YC, Huang SK. Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice. J Allergy Clin Immunol. 2007 Jun;119(6):1522-8. (RSG ELISA, TC sup, PubMed)

Manickan E, Smith JS, Tian J, Eggerman TL, Lozier JN, Muller J, Byrnes AP. Rapid Kupffer cell death after intravenous injection of adenovirus vectors. Mol Ther. 2006 Jan;13(1):108-17. (RSG ELISA, serum, PubMed)

Honma K, Udono H, Kohno T, Yamamoto K, Ogawa A, Takemori T, Kumatori A, Suzuki S, Matsuyama T, Yui K. Interferon regulatory factor 4 negatively regulates the production of proinflammatory cytokines by macrophages in response to LPS. Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16001-6. (RSG ELISA, serum, PubMed)