Mouse IL-12/IL-23 total p40 ELISA Ready-SET-Go!®

Also known as: Interleukin-12, IL12, IL23

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7120

Cat. No. Size
88-7120-22 2 x 96 tests w/plates
88-7120-76 20 x 96 tests w/plates
88-7120-77 20 x 96 tests
88-7120-86 10 x 96 tests w/plates
88-7120-88 10 x 96 tests

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Data for Mouse IL-12/IL-23 total p40 ELISA Ready-SET-Go!®.

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  • Data for Mouse IL-12/IL-23 total p40 ELISA Ready-SET-Go!®.
Description

Description: This Mouse IL-12 / IL-23 (Total p40) ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-12 / IL-23 (total p40) protein levels from samples including serum, plasma, and supernatants from cell cultures. This assay will detect p40 protein as a monomer, homodimer, or as a heterodimer of p35p40 (IL-12 p70) or p19p40 (IL-23). This assay is not allele-specific; it recognizes IL-12 p40 from ALL mouse strains tested (Ymer, SI., et al., Genes and Immunity, 2002, 3: 151-157).

Details
Reactivity Mouse
Sensitivity 2 pg/ml
Standard Curve Range 2 - 300 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References