Mouse IL-10 Instant ELISA

Also known as: TGIF, MCSF III, CSIF, B TCGF

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS614INST*

Cat. No. Size
BMS614INST 128 tests
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Mouse IL-10 Instant ELISA.

Click on image to view more information.

  • Data for Mouse IL-10 Instant ELISA.
Description

Description: The mouse IL-10 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-10. The mouse IL-10 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes.

The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Details
Reactivity Mouse
Sample Volume 50 uL
Suitable Sample Types serum, plasma (EDTA, citrate), cell culture supernatant
Sensitivity 5.28 pg/mL
Standard Curve Range 39.1 - 2,500 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-10, Biotin-Conjugate (anti-mouse IL-10 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminium pouch(es) with a mouse IL-10 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Sample Diluent (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Heinzel,F.P.; Sadick,M.D.; Mutha,S.S.; Locksley,R.M.. Production of interferon gamma, interleukin 2, interleukin 4, and interleukin 10 by CD4+ lymphocytes in vivo during healing and progressive murine leishmaniasis. Proc.Natl.Acad.Sci.U.S.A 1991;88:7011-7015. (Link)

Barnes,P.F.; Chatterjee,D.; Abrams,J.S.; Lu,S.; Wang,E.; Yamamura,M.; Brennan,P.J.; Modlin,R.L.. Cytokine production induced by Mycobacterium tuberculosis lipoarabinomannan. Relationship to chemical structure. J.Immunol. 1992;149:541-547. (Link)

Kim,J.M.; Brannan,C.I.; Copeland,N.G.; Jenkins,N.A.; Khan,T.A.; Moore,K.W.. Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes. J.Immunol. 1992;148:3618-3623. (Link)

Mosier,D.E.; Yetter,R.A.; Morse,H.C.,III. Retroviral induction of acute lymphoproliferative disease and profound immunosuppression in adult C57BL/6 mice. J.Exp.Med. 1985;161:766-784. (Link)

Basu,S.; Dunn,A.; Ward,A.. G-CSF: function and modes of action (Review). Int.J.Mol.Med. 2002;10:3-10. (Link)

Vieira,P.; de Waal-Malefyt,R.; Dang,M.N.; Johnson,K.E.; Kastelein,R.; Fiorentino,D.F.; deVries,J.E.; Roncarolo,M.G.; Mosmann,T.R.; Moore,K.W.. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc.Natl.Acad.Sci.U.S.A 1991;88:1172-1176. (Link)

Moore,K.W.; Vieira,P.; Fiorentino,D.F.; Trounstine,M.L.; Khan,T.A.; Mosmann,T.R.. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 1990;248:1230-1234. (Link)

Spits,H.; de Waal,Malefyt R.. Functional characterization of human IL-. Int.Arch.Allergy Immunol. 1992;99:8-15. (Link)

Kullberg,M.C.; Pearce,E.J.; Hieny,S.E.; Sher,A.; Berzofsky,J.A.. Infection with Schistosoma mansoni alters Th1/Th2 cytokine responses to a non-parasite antigen. J.Immunol. 1992;148:3264-3270. (Link)

de Waal,Malefyt R.; Abrams,J.; Bennett,B.; Figdor,C.G.; de Vries,J.E.. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J.Exp.Med. 1991;174:1209-1220. (Link)

Moore,K.W.; O'Garra,A.; de Waal,Malefyt R.; Vieira,P.; Mosmann,T.R.. Interleukin-10. Annu.Rev.Immunol. 1993;11:165-190. (Link)

Shanafelt,A.B.; Miyajima,A.; Kitamura,T.; Kastelein,R.A.. The amino-terminal helix of GM-CSF and IL-5 governs high affinity binding to their receptors. EMBO J. 1991;10:4105-4112. (Link)

Baer,R.; Bankier,A.T.; Biggin,M.D.; Deininger,P.L.; Farrell,P.J.; Gibson,T.J.; Hatfull,G.; Hudson,G.S.; Satchwell,S.C.; Seguin,C.; .. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 1984;310:207-211. (Link)

Silva,J.S.; Morrissey,P.J.; Grabstein,K.H.; Mohler,K.M.; Anderson,D.; Reed,S.G.. Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection. J.Exp.Med. 1992;175:169-174. (Link)

Bloom,B.R.; Mehra,V.. Immunological unresponsiveness in leprosy. Immunol.Rev. 1984;80:5-28. (Link)