Anti-Mouse IL-10 Alexa Fluor® 700

Also known as: Interleukin-10, IL10

Clone: JES5-16E3

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 56-7101

Cat. No. Size
56-7101-80 25 ug
56-7101-82 100 ug
Please inquire about custom configurations or bulk packaging.

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Data for Anti-Mouse IL-10 Alexa Fluor® 700.

Staining of restimulated cultured BALB/c splenocytes with Anti-Mouse CD4 FITC (cat. 11-0041)...View More

  • Data for Anti-Mouse IL-10 Alexa Fluor® 700.
Description

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Details
Host Rat
Isotype IgG2b, kappa
Reactivity Mouse
Conjugate Alexa Fluor 700
Laser Red Laser
Emit 723 nm
Excite 633 - 647 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
53-7101 Anti-Mouse IL-10 Alexa Fluor® 488 488 nm 519 nm FC RUO
14-7101 Anti-Mouse IL-10 Purified ELISA RUO
36-7101 Anti-Mouse IL-10 Functional Grade Biotin FA RUO
12-7101 Anti-Mouse IL-10 PE 488 - 561 nm 575 nm FC RUO
45-7101 Anti-Mouse IL-10 PerCP-Cyanine5.5 488 nm FC RUO
16-7101 Anti-Mouse IL-10 Functional Grade Purified ELISA, FA RUO
11-7101 Anti-Mouse IL-10 FITC 488 nm 518 nm FC RUO
17-7101 Anti-Mouse IL-10 APC 633 - 647 nm 660 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0041 Anti-Mouse CD4 FITC 488 nm 518 nm FC RUO
56-4031 Rat IgG2b K Isotype Control Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
References

References: Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 1662: 201-14.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.