Anti-Mouse IL-10 Alexa Fluor® 488

Also known as: Interleukin-10, IL10

Clone: JES5-16E3

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-7101

Cat. No.	Size
53-7101-80	25 ug
53-7101-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Intracellular staining of Mouse Cytokine Positive Control Cells (cat. 00-4500) with 0.25 ug of ...View More

Description

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
11-7101	Anti-Mouse IL-10 FITC	488 nm	518 nm	FC	RUO
36-7101	Anti-Mouse IL-10 Functional Grade Biotin			FA	RUO
12-7101	Anti-Mouse IL-10 PE	488 - 561 nm	575 nm	FC	RUO
14-7101	Anti-Mouse IL-10 Purified			ELISA	RUO
45-7101	Anti-Mouse IL-10 PerCP-Cyanine5.5	488 nm		FC	RUO
17-7101	Anti-Mouse IL-10 APC	633 - 647 nm	660 nm	FC	RUO
16-7101	Anti-Mouse IL-10 Functional Grade Purified			ELISA, FA	RUO
56-7101	Anti-Mouse IL-10 Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO

Related Products

Cat. No.	Name	Application	Reg.
00-4500	Mouse Cytokine Positive Control Cells	FC	RUO
13-7102	Anti-Mouse IL-10 Biotin	ELISA	RUO
16-7102	Anti-Mouse IL-10 Functional Grade Purified	FA	RUO
88-7904	Mouse IL-10 (Interleukin-10, IL10) ELISA Ready-SET-Go! Kit (See replacement item 88-7104)	ELISA	RUO

References

References: Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 1662: 201-14.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

View More Products

Antigens	Area of Biology	Cell Type	Formats
IL-10	Adaptive Immunity Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells T Cells Treg Cells Th2 Cells	Alexa Fluor 488