Anti-Mouse IgD antiserum

Description: The anti-mouse IgD polyclonal goat antiserum reacts with the δ heavy chain of mouse IgD, and is a useful polyclonal activator of B cells (in vitro or in vivo). IgD is expressed by mature peripheral B cells. Unlike most monoclonal antibodies specific for IgD, this goat polyclonal anti-IgD antiserum is a strong activator of B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic by virtue of interference by the high levels of circulating serum IgM. This goat polyclonal anti-IgD antiserum is useful for in vivo induction of IgE(1) and IL-4/Th2(2) responses.

References: Finkelman F, Snapper C, Mountz J, and Katona I. 1987. Polyclonal activation of the murine immune system by a goat antibody to mouse IgD. J. Immunol. 138: 2826-2830.

Finkelman F, Morris S, Orekhova T, and Sehy D. 2003. The Cincinnati Cytokine Capture Assay (CCCA) for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, and Strober W, eds. Greene Publishing Associates and Wiley-Interscience, New York, Unit 6.28, supplement 54.

Choo, S-W, and DH Conrad. 1997. A new multivalent B cell activation model - anti-IgD bound to FcgRI: properties and comparison with CD40L-mediated activation. International Immunol. 9: 239-248.