Mouse Th1/Th2 ELISA Ready-SET-Go!®

Also known as: IFNg, IL-10, IL-2, IL-4

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7711

Cat. No. Size
88-7711-44 Panel
Please inquire about custom configurations or bulk packaging.

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Data for Mouse Th1/Th2 ELISA Ready-SET-Go!®.

Description

Description: The eBioscience Mouse Th1/Th2 ELISA Ready-SET-Go! contains 4 pairs of antibodies that can be used to discriminate Th1/Th2 subsets based on their expression of IFN-γ, IL-2, IL-4 and IL-10. This panel consists of sufficient amounts of reagents to run two 96-well ELISA plate assays for each of these four cytokines. This ELISA reagent set is specifically engineered for accurate and precise measurement from samples including serum, plasma, and supernatants from cell cultures.

Special Note: To ensure optimal results from the Mouse Th1/Th2 ELISA Ready-SET-Go! Set, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

Details
Reactivity Mouse
Sensitivity Mouse IFN-gamma: 15 pg/ml IL-2: 2 pg/ml IL-4: 4 pg/ml IL-10: 30 pg/ml
Standard Curve Range Mouse IFN-gamma: 15 - 2000 pg/ml IL-2: 2 - 200 pg/ml IL-4: 4 - 500 pg/ml IL-10: 30 - 4000 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Onishi N, Kawamoto S, Suzuki H, Santo H, Aki T, Shigeta S, Hashimoto K, Hide M, Ono K. Dietary pulverized konjac glucomannan suppresses scratching behavior and skin inflammatory immune responses in NC/Nga mice. Int Arch Allergy Immunol. 2007;144(2):95-104. (RSG ELISA kit, tissue homogenate, PubMed)

Augello A, Tasso R, Negrini SM, Cancedda R, Pennesi G. Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis. Arthritis Rheum. 2007 Apr;56(4):1175-86. (RSG ELISA kit, serum, PubMed)