Mouse IFN-gamma Instant ELISA

Description: The mouse IFN-gamma Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of murine Interferon gamma. The mouse IFN-gamma Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

IFN-gamma, also called Type II interferon, is a homodimeric gycoprotein containing approximately 21 to 24 kD subunits.

In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. All IFN-gamma inducers activate T cells either in a polyclonal (mitogens or antibodies) or in a clonally restricted, antigen-specific, manner.
IFN-gamma is produced during infection by T cells of the cytotxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNF-beta and IFN-gamma, whereas Th2 cells mainly produce IL-3, IL-4, IL-5 and IL-10, but little or no IFN-gamma. IFN-gamma preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFN-gamma during an immune response will result in the preferential proliferation of Th1 cells.

Type II IFN or IFN-gamma is a lymphokine that displays no molecular homology with type I IFN, but shares some important biologic activities. Specifically, IFN-gamma induces an anti-viral state and is anti-proliferative. In addition, IFN-gamma has several properties related to immunoregulation.

1) IFN-gamma is a potent activator of mononuclear phagocytes, e.g. IFN-gamma stimulates the expression of Mac-1, augments endocytosis and phagocytosis by monocytes; and activates macrophages to kill tumor cells by releasing reactive oxagen intermediates and TNF-alpha.

2) IFN-gamma induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines.

3) On T and B cells IFN-gamma promotes differentiation. It enhaces proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis. IFN-gamma is one of the natural B-cell differentiation factors.

4) Finally, IFN-gamma activates neutrophils, NK cells and vascular endothelial cells.

The role of IFN-gamma as a disease marker has been demonstrated for a number of different pathological situations

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