Anti-Human/Mouse GL7 (T and B Cell Activation Marker) eFluor® 450

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

References: Kimura N, Ohmori K, Miyazaki K, Izawa M, Matsuzaki Y, Yasuda Y, Takematsu H, Kozutsumi Y, Moriyama A, Kannagi R. Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2. J Biol Chem. 2007 Nov 2;282(44):32200-7. (GL7, FA, human cells, PubMed)

Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, Sugai M, Okuno Y, Tsujimoto G, Yamaji T, Hashimoto Y, Itohara S, Kawasaki T, Suzuki A, Kozutsumi Y. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation. Mol Cell Biol. 2007 Apr;27(8):3008-22.

Han, S., B. Zheng, Y. Takahashi, and G. Kelsoe. Distinctive characteristics of germinal center B cells. Semin. Immunol. 1997; 9: 255 - 260.

Han, S., S.R. Dillon, B. Zheng, M. Shimoda, M.S. Schlissel, and G. Kelsoe. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science 1997; 278: 301 - 305.

Han, S., B. Zheng, D.G. Schatz, E. Spanopoulou, and G. Kelsoe. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science 1996; 274: 2094 - 2097.

Hathcock, K.S., C.E.M. Pucillo, G. Laszlo, L. Lai, and R.J. Hodes. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J. Immunol. 1995; 155: 4575 - 4581.

Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 1993; 150: 5252 - 5262.