Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Alexa Fluor® 647

Also known as: GL-7, Ly-77, Ly77

Clone: GL-7 (GL7)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 51-5902

Cat. No.	Size
51-5902-80	25 ug
51-5902-82	100 ug

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Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

Details

Host	Rat
Isotype	IgM
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-5902	Anti-Human/Mouse GL7 (T and B Cell Activation Marker) eFluor® 450	405 nm	450 nm	FC	RUO
53-5902	Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Alexa Fluor® 488	488 nm	519 nm	FC	RUO
14-5902	Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified			FC, IHC, IP	RUO
13-5902	Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Biotin			FC	RUO

References

References: Kimura N, Ohmori K, Miyazaki K, Izawa M, Matsuzaki Y, Yasuda Y, Takematsu H, Kozutsumi Y, Moriyama A, Kannagi R. Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2. J Biol Chem. 2007 Nov 2;282(44):32200-7. (GL7, FA, human cells, PubMed)

Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, Sugai M, Okuno Y, Tsujimoto G, Yamaji T, Hashimoto Y, Itohara S, Kawasaki T, Suzuki A, Kozutsumi Y. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation.Mol Cell Biol. 2007 Apr;27(8):3008-22.

Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252 - 5262.

Han, S., B. Zheng, D.G. Schatz, E. Spanopoulou, and G. Kelsoe. 1996. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science 274:2094 - 2097.

Han, S., B. Zheng, Y. Takahashi, and G. Kelsoe. 1997. Distinctive characteristics of germinal center B cells. Semin. Immunol. 9: 255 - 260.

Han, S., S.R. Dillon, B. Zheng, M. Shimoda, M.S. Schlissel, and G. Kelsoe. 1997. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science278: 301 - 305.

Hathcock, K.S., C.E.M. Pucillo, G. Laszlo, L. Lai, and R.J. Hodes. 1995. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J. Immunol. 155: 4575 - 4581.

View More Products

Antigens	Area of Biology	Cell Type	Formats
GL7 (T and B Cell Activation Marker)	Adaptive Immunity	B Cells T Cells	Alexa Fluor 647