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Anti-Mouse GARP eFluor® 450

Also known as: Lrrc32, Garpin

Clone: YGIC86

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 48-9891

Cat. No. Size
48-9891-80 25 ug
48-9891-82 100 ug

Data for Anti-Mouse GARP eFluor® 450 (Pacific Blue® replacement).

BALB/c splenocytes were stimulated with Anti-Mouse CD3 Functional Grade Purified (cat. 16-0031),...View More

  • Data for Anti-Mouse GARP eFluor® 450 (Pacific Blue® replacement).
Description

Description: The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin). GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta.

When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody. Fixation/permeabilization is not necessary when staining Treg cells.

YGIC86 does not cross-react to rat GARP.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Mouse
Conjugate eFluor 450
Laser Violet Laser
Emit 445 nm
Excite 405 nm
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-9891 Anti-Mouse GARP PE 488 - 561 nm 578 nm FC RUO
17-9891 Anti-Mouse GARP APC 633 - 647 nm 660 nm FC RUO
25-9891 Anti-Mouse GARP PE-Cyanine7 488 - 561 nm 775 nm FC RUO
46-9891 Anti-Mouse GARP PerCP-eFluor® 710 488 nm 710 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
14-8021 Mouse IL-2 Recombinant Protein FA RUO
16-0281 Anti-Mouse CD28 Functional Grade Purified FC, FA RUO
48-4321 Rat IgG2a K Isotype Control eFluor® 450 405 nm 445 nm FC RUO
11-0042 Anti-Mouse CD4 FITC 488 nm 520 nm FC RUO
16-0031 Anti-Mouse CD3e Functional Grade Purified FC, FA RUO
17-5773 Anti-Mouse/Rat Foxp3 APC 633 - 647 nm 660 nm FC RUO
References

References: Ujiie H, Shevach EM. Gamma-Delta T Cells Protect the Liver and Lungs of Mice from Autoimmunity Induced by Scurfy Lymphocytes. J Immunol. 2016 Feb 15;196(4):1517-28. (YGIC86, FC, PubMed)

Rezende RM, da Cunha AP, Kuhn C, Rubino S, M'Hamdi H, Gabriely G, Vandeventer T, Liu S, Cialic R, Pinheiro-Rosa N, Oliveira RP, Gaublomme JT, Obholzer N, Kozubek J, Pochet N, Faria AM, Weiner HL. Identification and characterization of latency-associated peptide-expressing gamma-delta T cells. Nat Commun. 2015 Dec 8;6:8726. (YGIC86, FC, PubMed)

Carrillo-Galvez AB, Cobo M, Cuevas-Ocaña S, Gutiérrez-Guerrero A, Sánchez-Gilabert A, Bongarzone P, García-Pérez A, Muñoz P, Benabdellah K, Toscano MG, Martín F, Anderson P. Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity. Stem Cells. 2015 Jan;33(1):183-95. (YGIC86, FC, PubMed)

Oida T, Weiner HL. TGF-beta induces surface LAP expression on murine CD4 T cells independent of Foxp3 induction. PLoS One. 2010 Nov 24;5(11):e15523. (YGIC86, FC, PubMed)

Oida T, Weiner HL. Overexpression of TGF-beta 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-beta. J Immunol. 2010 Sep 15;185(6):3529-35. (YGIC86, FC, PubMed)

Wang R, Kozhaya L, Mercer F, Khaitan A, Fujii H, Unutmaz D. Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells. Proc Natl Acad Sci USA. 2009 Aug 11;106(32):13439-44.

Tran DQ, Andersson J, Wang R, Ramsey H, Unutmaz D, Shevach EM. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci USA. 2009 Aug 11;106(32):13445-50.

Wang R, Wan Q, Kozhaya L, Fujii H, Unutmaz D. Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression. PLoS One. 2008 Jul 16;3(7):e2705.

Macaulay IC, Tijssen MR, Thijssen-Timmer DC, Gusnanto A, Steward M, Burns P, Langford CF, Ellis PD, Dudbridge F, Zwaginga JJ, Watkins NA, van der Schoot CE, Ouwehand WH. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins. Blood. 2007 Apr 15;109(8):3260-9.

Roubin R, Pizette S, Ollendorff V, Planche J, Birnbaum D, Delapeyriere O. Structure and developmental expression of mouse Garp, a gene encoding a new leucine-rich repeat-containing protein. Int J Dev Biol. 1996 Jun;40(3):545-55.

Ollendorff V, Noguchi T, deLapeyriere O, Birnbaum D. The GARP gene encodes a new member of the family of leucine-rich repeat-containing proteins. Cell Growth Differ. 1994 Feb;5(2):213-9.


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