Mouse Eotaxin Platinum ELISA

Description: The mouse Eotaxin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse Eotaxin. The mouse Eotaxin ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Eotaxin is a member of the CC chemokine family of inflammatory and immunoregulatory cytokines. Constitutive Eotaxin mRNA expression has been detected in multiple tissues, most often it appears to be induced by inflammatory cytokines such as IL-1, TNF-alpha and IFN-gamma in fibroblasts, endothelial and epithelial cells. Following allergen challenge Eotaxin mRNA has been shown to be rapidly up-regulated in airway epithelium an alveolar macrophages. Eotaxin expression has also been detected in smooth muscle cells, chondrocytes and eosinophils. Mouse Eotaxin activity is mediated by the mouse CC chemokine receptor CCR3, which, unlike human CCR3, can also be activated by mouse MIP-1alpha. Among CC chemokine familiy members, Eotaxin is functionally and structurally mostly related to the MCP/Eotaxin proteins. Mouse Eotaxin cDNA encodes a 97 amino acid residue precursor protein that is cleaved to generate the 74 aa residue mature protein. Mouse Eotaxin has been shown to be a potent chemoattractant for eosinophils during inflammation and allergic reactions and to be involved in the growth of myeloid cell progenitors and the differentiation of mast cells during embryonic development. A role in numerous eosinophil-associated gastrointestinals disorders as food allergy, parasitic infections, allergic colitis and inflammatory Bowel disease has been described.

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Ponath,P.D.; Qin,S.; Ringler,D.J.; Clark-Lewis,I.; Wang,J.; Kassam,N.; Smith,H.; Shi,X.; Gonzalo,J.A.; Newman,W.; Gutierrez-Ramos,J.C.; Mackay,C.R.. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils 99. J.Clin.Invest 1996;97:604-612. (Link)

Quackenbush,E.J.; Aguirre,V.; Wershil,B.K.; Gutierrez-Ramos,J.C.. Eotaxin influences the development of embryonic hematopoietic progenitors in the mouse. J.Leukoc.Biol. 1997;62:661-666. (Link)

Luster,A.D.; Rothenberg,M.E.. Role of the monocyte chemoattractant protein and eotaxin subfamily of chemokines in allergic inflammation. J.Leukoc.Biol. 1997;62:620-633. (Link)

Li,D.; Wang,D.; Griffiths-Johnson,D.A.; Wells,T.N.; Williams,T.J.; Jose,P.J.; Jeffery,P.K.. Eotaxin protein and gene expression in guinea-pig lungs: constitutive expression and upregulation after allergen challenge. Eur.Respir.J. 1997;10:1946-1954. (Link)

Post,T.W.; Bozic,C.R.; Rothenberg,M.E.; Luster,A.D.; Gerard,N.; Gerard,C.. Molecular characterization of two murine eosinophil beta chemokine receptors. J.Immunol. 1995;155:5299-5305. (Link)

Lilly,C.M.; Nakamura,H.; Kesselman,H.; Nagler-Anderson,C.; Asano,K.; Garcia-Zepeda,E.A.; Rothenberg,M.E.; Drazen,J.M.; Luster,A.D.. Expression of eotaxin by human lung epithelial cells: induction by cytokines and inhibition by glucocorticoids. J.Clin.Invest 1997;99:1767-1773. (Link)

Bartels,J.; Schluter,C.; Richter,E.; Noso,N.; Kulke,R.; Christophers,E.; Schroder,J.M.. Human dermal fibroblasts express eotaxin: molecular cloning, mRNA expression, and identification of eotaxin sequence variants 12. Biochem.Biophys.Res.Commun. 1996;225:1045-1051. (Link)