Anti-Mouse EOMES Alexa Fluor® 647 (To Be Discontinued; refer to Cat. No. 50-4875)

Also known as: Eomesodermin, TBR2

Clone: Dan11mag

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Replacement format is eFluor 660. Select Cat. No. 50-4875

SKU# 51-4875

Cat. No.	Size
51-4875-80	25 ug
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Surface staining of C57BL/6 splenocytes with Anti-Mouse NK1.1 PE (cat. 12-5941) followed by...View More

Description

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 - 647 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-4875	Anti-Mouse EOMES Purified			FC, WB	RUO
48-4875	Anti-Mouse EOMES eFluor® 450	405 nm		FC	RUO
12-4875	Anti-Mouse EOMES PE	488 - 561 nm		FC	RUO
50-4875	Anti-Mouse EOMES eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
25-4875	Anti-Mouse EOMES PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
46-4875	Anti-Mouse EOMES PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
53-4875	Anti-Mouse EOMES Alexa Fluor® 488	488 nm	519 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5521	Foxp3 Fixation/Permeabilization Concentrate and Diluent			FC	GPR
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
11-0031	Anti-Mouse CD3e FITC	488 nm		FC	RUO
12-5941	Anti-Mouse NK1.1 PE	488 - 561 nm	575 nm	FC	RUO
51-4321	Rat IgG2a K Isotype Control Alexa Fluor® 647 (To Be Discontinued. Refer to Cat. No. 50-4321)	633 - 647 nm	668 nm	FC, ICC, IHC	RUO

References

References: Weinreich MA, Odumade OA, Jameson SC, Hogquist KA T cells expressing the transcription factor PLZF regulate the development of memory-like CD8+ T cells. Nat Immunol. 2010 Aug;11(8):709-16 (Dan11mag, IC flow, PubMed)

Marshall HD, Prince AL, Berg LJ, Welsh RM. IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions. J Immunol. 2010 Aug 1;185(3):1419-28. (Dan11mag, IC flow, PubMed)

Intlekofer AM, Banerjee A, Takemoto N, Gordon SM, Dejong CS, Shin H, Hunter CA, Wherry EJ, Lindsten T, Reiner SL. Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin. Science. 2008 Jul 18;321(5887):408-11.

Intlekofer AM, Takemoto N, Wherry EJ, Longworth SA, Northrup JT, Palanivel VR, Mullen AC, Gasink CR, Kaech SM, Miller JD, Gapin L, Ryan K, Russ AP, Lindsten T, Orange JS, Goldrath AW, Ahmed R, Reiner SL. Effector and memory CD8+ T cell fate coupled by T-bet and eomesodermin. Nat Immunol. 2005 Dec;6(12):1236-44.

Pearce EL, Mullen AC, Martins GA, Krawczyk CM, Hutchins AS, Zediak VP, Banica M, DiCioccio CB, Gross DA, Mao CA, Shen H, Cereb N, Yang SY, Lindsten T, Rossant J, Hunter CA, Reiner SL. Control of effector CD8+ T cell function by the transcription factor Eomesodermin. Science. 2003 Nov 7;302(5647):1041-3.

Russ AP, Wattler S, Colledge WH, Aparicio SA, Carlton MB, Pearce JJ, Barton SC, Surani MA, Ryan K, Nehls MC, Wilson V, Evans MJ. Eomesodermin is required for mouse trophoblast development and mesoderm formation. Nature. 2000 Mar 2;404(6773):95-9.

Ciruna BG, Rossant J. Expression of the T-box gene Eomesodermin during early mouse development. Mech Dev. 1999 Mar;81(1-2):199-203.

Hancock SN, Agulnik SI, Silver LM, Papaioannou VE. Mapping and expression analysis of the mouse ortholog of Xenopus Eomesodermin. Mech Dev. 1999 Mar;81(1-2):205-8.

Ryan K, Garrett N, Mitchell A, Gurdon JB. Eomesodermin, a key early gene in Xenopus mesoderm differentiation. Cell. 1996 Dec 13;87(6):989-1000.

View More Products

Antigens	Area of Biology	Cell Type	Formats
Eomes	Adaptive Immunity Innate Immunity Transcription Factors	NK & NKT Cells T Cells	Alexa Fluor 647