Anti-Mouse Egr2 PE

Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.

References: Iwasaki Y, Fujio K, Okamura T, Yanai A, Sumitomo S, Shoda H, Tamura T, Yoshida H, Charnay P, Yamamoto K. Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4(+) T cells. Eur J Immunol. 2013 Jan 25. doi: 10.1002/eji.201242942 (erongr2, FC, PubMed)

Seiler MP, Mathew R, Liszewski MK, Spooner C, Barr K, Meng F, Singh H, Bendelac A. Elevated and sustained expression of the transcription factors Egr1 and Egr2 controls NKT lineage differentiation in response to TCR signaling. Nat Immunol. 2012 Feb 5;13(3):264-71. (erongr2, FC, PubMed)

Lawson VJ, Weston K, Maurice D. Early growth response 2 regulates the survival of thymocytes during positive selection. Eur J Immunol. 2010 Jan;40(1):232-41.

Lazarevic V, Zullo AJ, Schweitzer MN, Staton TL, Gallo EM, Crabtree GR, Glimcher LH. The gene encoding early growth response 2, a target of the transcription factor NFAT, is required for the development and maturation of natural killer T cells. Nat Immunol. 2009 Mar;10(3):306-13.

Safford M, Collins S, Lutz MA, Allen A, Huang CT, Kowalski J, Blackford A, Horton MR, Drake C, Schwartz RH, Powell JD. Egr-2 and Egr-3 are negative regulators of T cell activation. Nat Immunol. 2005 May;6(5):472-80.

Carleton M, Haks MC, Smeele SA, Jones A, Belkowski SM, Berger MA, Linsley P, Kruisbeek AM, Wiest DL. Early growth response transcription factors are required for development of CD4(-)CD8(-) thymocytes to the CD4(+)CD8(+) stage. J Immunol. 2002 Feb 15;168(4):1649-58.

Mack KJ, Cortner J, Mack P, Farnham PJ. Krox 20 messenger RNA and protein expression in the adult central nervous system. Brain Res Mol Brain Res. 1992 Jun;14(1-2):117-23.