Anti-Mouse DLL1 (delta-like 1) Alexa Fluor® 488

Also known as: DL1

Clone: HMD1-5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-5767

Cat. No. Size
53-5767-80 25 ug
53-5767-82 100 ug
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.


Data for Anti-Mouse DLL1 (delta-like 1) Alexa Fluor® 488.

Staining of CD8+CD11c+ gated C57BL/6 splenocytes with 0.5 ug of Armenian Hamster IgG Isotype Control...View More

  • Data for Anti-Mouse DLL1 (delta-like 1) Alexa Fluor® 488.
Description

Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by γ-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis.

Details
Host Armenian Hamster
Isotype IgG
Reactivity Mouse
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
16-5767 Anti-Mouse DLL1 (delta-like 1) Functional Grade Purified FA RUO
12-5767 Anti-Mouse DLL1 (delta-like 1) PE 488 - 561 nm 578 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
12-0081 Anti-Mouse CD8a PE 488 - 561 nm 578 nm FC RUO
53-4888 Armenian Hamster IgG Isotype Control Alexa Fluor® 488 488 nm 519 nm FC, ICC, IHC RUO
17-0114 Anti-Mouse CD11c APC 633 - 647 nm 660 nm FC RUO
References

References: Sekine C, Moriyama Y, Koyanagi A, Koyama N, Ogata H, Okumura K, Yagita H. Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int Immunol. 2009 Jan 30. (HMD1-5, FC, Pubmed)

Moriyama Y, Sekine C, Koyanagi A, Koyama N, Ogata H, Chiba S, Hirose S, Okumura K, Yagita H. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int Immunol. 2008 Jun;20(6):763-73. (HMD1-5, FC, Pubmed)

Mohtashami M, Zúñiga-Pflücker JC. Three-dimensional architecture of the thymus is required to maintain delta-like expression necessary for inducing T cell development. J Immunol. 2006 Jan 15;176(2):730-4.

Hozumi K, Negishi N, Suzuki D, Abe N, Sotomaru Y, Tamaoki N, Mailhos C, Ish-Horowicz D, Habu S, Owen MJ. Delta-like 1 is necessary for the generation of marginal zone B cells but not T cells in vivo. Nat Immunol. 2004 Jun;5(6):638-44.