Mouse CXCL1/KC FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Mouse CXCL1/KC in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Mouse Basic Kit (cat. BMS8440FF), this kit can be used to detect CXCL1/KC alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A5.

The Keratinocyte chemoattractant gene is a member of the small inducible gene family of regulated chemokines. It encodes a growth factor and chemoattractant that appears to mediate inflammatory and immune responses in vitro and in vivo.
Chemokine KC (CXCL1) has been considered to be a murine homologue of human GRO/MGSA and was identified as chemoattractant for monocytes and neutrophils.

Increased KC expression has been found to be associated with neutrophil influx in various inflammatory conditions. An important role for KC/CXCL1 has been shown in experimental model of multiple sclerosis as a chemoattractant of destructive immune cells. Data further indicate that KC/GRO chemokines are the principal chemokines induced by LPS and pro-inflammatory cytokines IL-1 and TNF via NFkappaB signalling in ovarian surface epithelial cancer cells.

References: Oquendo,P.; Alberta,J.; Wen,D.Z.; Graycar,J.L.; Derynck,R.; Stiles,C.D.. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins. J Biol Chem. 1989;264:4133-4137. (Link)

Kim,H.S.; Shin,D.H.; Kim,S.K.. Effects of interleukin-10 on chemokine KC gene expression by mouse peritoneal macrophages in response to Candida albicans. J Korean Med Sci. 1999;14:480-486. (Link)

Kim,J.M.; Kim,Y.J.; Cho,Y.J.. Synergy of Bacteroides fragilis and Escherichia coli in the induction of KC gene expression in mouse peritoneal tissues. Scand.J Infect.Dis 2000;32:643-649. (Link)

Deng,Z.W.; Denkinger,D.J.; Peterson,K.E.; Deuel,T.F.; Kawahara,R.S.. Glucocorticoids negatively regulate the transcription of KC, the mouse homolog of MGSA/GRO. Biochem.Biophys.Res.Commun. 1994;203:1809-1814. (Link)

Heesen,M.; Tanabe,S.; Berman,M.A.; Yoshizawa,I.; Luo,Y.; Kim,R.J.; Post,T.W.; Gerard,C.; Dorf,M.E.. Mouse astrocytes respond to the chemokines MCP-1 and KC, but reverse transcriptase-polymerase chain reaction does not detect mRNA for the KC or new MCP-1 receptor. J Neurosci.Res. 1996;45:382-391. (Link)

Kim,H.Y.; Kim,H.K.; Kim,J.R.; Kim,H.S.. Upregulation of LPS-induced chemokine KC expression by 15-deoxy-delta12,14-prostaglandin J2 in mouse peritoneal macrophages. Immunol.Cell Biol 2005;83:286-293. (Link)

Son,D.S.; Parl,A.K.; Rice,V.M.; Khabele,D.. Keratinocyte chemoattractant (KC)/human growth-regulated oncogene (GRO) chemokines and pro-inflammatory chemokine networks in mouse and human ovarian epithelial cancer cells. Cancer Biol Ther. 2007;6:1302-1312. (Link)

Tebo,J.M.; Datta,S.; Kishore,R.; Kolosov,M.; Major,J.A.; Ohmori,Y.; Hamilton,T.A.. Interleukin-1-mediated stabilization of mouse KC mRNA depends on sequences in both 5'- and 3'-untranslated regions. J Biol Chem. 2000;275:12987-12993. (Link)

Lira,S.A.; Zalamea,P.; Heinrich,J.N.; Fuentes,M.E.; Carrasco,D.; Lewin,A.C.; Barton,D.S.; Durham,S.; Bravo,R.. Expression of the chemokine N51/KC in the thymus and epidermis of transgenic mice results in marked infiltration of a single class of inflammatory cells. J Exp.Med 1994;180:2039-2048. (Link)

Rubio,N.; Sanz-Rodriguez,F.. Induction of the CXCL1 (KC) chemokine in mouse astrocytes by infection with the murine encephalomyelitis virus of Theiler. Virology 2007;358:98-108. (Link)

Nardini,E.; Morelli,D.; Aiello,P.; Besusso,D.; Calcaterra,C.; Mariani,L.; Palazzo,M.; Vecchi,A.; Paltrinieri,S.; Menard,S.; Balsari,A.. CpG-oligodeoxynucleotides induce mobilization of hematopoietic progenitor cells into peripheral blood in association with mouse KC (IL-8) production. J Cell Physiol 2005;204:889-895. (Link)

Wuyts,A.; Haelens,A.; Proost,P.; Lenaerts,J.P.; Conings,R.; Opdenakker,G.; Van,Damme J.. Identification of mouse granulocyte chemotactic protein-2 from fibroblasts and epithelial cells. Functional comparison with natural KC and macrophage inflammatory protein-2. J Immunol. 1996;157:1736-1743. (Link)

Ohmori,Y.; Hamilton,T.A.. IFN-gamma selectively inhibits lipopolysaccharide-inducible JE/monocyte chemoattractant protein-1 and KC/GRO/melanoma growth-stimulating activity gene expression in mouse peritoneal macrophages. J Immunol. 1994;153:2204-2212. (Link)

Hanazawa,S.; Murakami,Y.; Takeshita,A.; Kitami,H.; Ohta,K.; Amano,S.; Kitano,S.. Porphyromonas gingivalis fimbriae induce expression of the neutrophil chemotactic factor KC gene of mouse peritoneal macrophages: role of protein kinase C. Infect.Immun. 1992;60:1544-1549. (Link)

Ohmori,Y.; Fukumoto,S.; Hamilton,T.A.. Two structurally distinct kappa B sequence motifs cooperatively control LPS-induced KC gene transcription in mouse macrophages. J Immunol. 1995;155:3593-3600. (Link)

Luo,Y.; Fischer,F.R.; Hancock,W.W.; Dorf,M.E.. Macrophage inflammatory protein-2 and KC induce chemokine production by mouse astrocytes. J Immunol. 2000;165:4015-4023. (Link)