Anti-Mouse Clec9A PerCP-eFluor® 710

Description: This 42D2 monoclonal antibody reacts with mouse Clec9A, which is also known as DNGR-1. Clec9A is a C-type lectin-like receptor expressed on conventional and plasmacytoid dendritic cells (DCs). In particular, CD8+ conventional DCs in the thymus, spleen, and lymph nodes, as well as CD24+ DCs in the blood, express Clec9A. This receptor is not detectable on most B cells, T and NK cells, monocytes, and macrophages. The cytoplasmic domain of Clec9A contains an ITAM motif that has been demonstrated to phosphorylate Syk tyrosine kinase, leading to NFAT activation. This signaling reportedly mediates the presentation of necrotic cell-associated antigens by CD8+ DCs in innate immunity.

References: Sancho D, Joffre OP, Keller AM, Rogers NC, Martínez D, Hernanz-Falcón P, Rosewell I, Reis e Sousa C. Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature. 2009 Apr 16;458(7240):899-903.

Caminschi I, Proietto AI, Ahmet F, Kitsoulis S, Shin Teh J, Lo JC, Rizzitelli A, Wu L, Vremec D, van Dommelen SL, Campbell IK, Maraskovsky E, Braley H, Davey GM, Mottram P, van de Velde N, Jensen K, Lew AM, Wright MD, Heath WR, Shortman K, Lahoud MH. The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008 Oct 15;112(8):3264-73.

Huysamen C, Willment JA, Dennehy KM, Brown GD. CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem. 2008 Jun 13;283(24):16693-701.