Anti-Mouse CD8b Alexa Fluor® 488

Also known as: CD8 beta, Ly-3, Ly-C, Lyt-3

Clone: eBioH35-17.2 (H35-17.2)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0083

Cat. No.	Size
53-0083-81	50 ug
53-0083-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of BALB/c splenocytes with Anti-Mouse CD8a APC (cat. 17-0081) and 0.125 ug of Rat IgG2b K...View More

Description

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-0083	Anti-Mouse CD8b eFluor® 450	405 nm	450 nm	FC	RUO
13-0083	Anti-Mouse CD8b Biotin			FC	RUO
15-0083	Anti-Mouse CD8b PE-Cyanine5	488 - 561 nm		FC	RUO
12-0083	Anti-Mouse CD8b PE	488 - 561 nm	575 nm	FC	RUO
14-0083	Anti-Mouse CD8b Purified			FC, IHC, IP	RUO
11-0083	Anti-Mouse CD8b FITC	488 nm	518 nm	FC	RUO
17-0083	Anti-Mouse CD8b APC	633 - 647 nm	660 nm	FC	RUO
46-0083	Anti-Mouse CD8b PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
25-0083	Anti-Mouse CD8b PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
17-0081	Anti-Mouse CD8a APC	633 - 647 nm	660 nm	FC	RUO
53-4031	Rat IgG2b K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991 Sep 15;147(6):1746-51. (H35-17.2, FC, PubMed)

Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981 Jun 1;153(6):1503-16.

Golstein P, Goridis C, Schmitt-Verhulst AM, Hayot B, Pierres A, van Agthoven A, Kaufmann Y, Eshhar Z, Pierres M. Lymphoid cell surface interaction structures detected using cytolysis-inhibiting monoclonal antibodies. Immunol Rev. 1982;68:5-42. Review.

Tarleton RL, Sun J, et al. 1994. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun. 62(5):1820-9. (H35-17.2, IHC frozen, PubMed)

Thoma-Uszynski S, Emoto M, and Kaufmann SH. 1997. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog. 23(2):101-6. (H35-17.2, IHC frozen, PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD8b	Adaptive Immunity	NK & NKT Cells T Cells	Alexa Fluor 488