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Anti-Mouse CD8a Super Bright 600

Also known as: CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2

Clone: 53-6.7

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 63-0081

Cat. No. Size
63-0081-80 25 ug
63-0081-82 100 ug

Data for Anti-Mouse CD8a Super Bright 600.

Staining of mouse splenocytes with Anti-Mouse CD3 APC (cat. 17-0032) and 0.25 ug of Rat IgG2a K...View More

  • Data for Anti-Mouse CD8a Super Bright 600.
Description

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Mouse
Conjugate Super Bright 600
Laser Violet Laser
Emit 600 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
45-0081 Anti-Mouse CD8a PerCP-Cyanine5.5 488 nm 695 nm FC RUO
14-0081 Anti-Mouse CD8a Purified FC, IHC, IHC-F, IP RUO
47-0081 Anti-Mouse CD8a APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
46-0081 Anti-Mouse CD8a PerCP-eFluor® 710 488 nm 710 nm FC RUO
53-0081 Anti-Mouse CD8a Alexa Fluor® 488 488 nm 519 nm FC RUO
48-0081 Anti-Mouse CD8a eFluor® 450 405 nm 445 nm FC RUO
25-0081 Anti-Mouse CD8a PE-Cyanine7 488 - 561 nm 775 nm FC RUO
11-0081 Anti-Mouse CD8a FITC 488 nm 520 nm FC RUO
12-0081 Anti-Mouse CD8a PE 488 - 561 nm 578 nm FC RUO
35-0081 Anti-Mouse CD8a PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
13-0081 Anti-Mouse CD8a Biotin FC, IHC, IHC-F RUO
17-0081 Anti-Mouse CD8a APC 633 - 647 nm 660 nm FC RUO
36-0081 Anti-Mouse CD8a Functional Grade Biotin FC, IHC, IHC-F RUO
58-0081 Anti-Mouse CD8a Alexa Fluor® 532 532 nm 561 nm FC RUO
56-0081 Anti-Mouse CD8a Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
50-0081 Anti-Mouse CD8a eFluor® 660 633 - 647 nm 668 nm FC, IHC, IHC-F RUO
15-0081 Anti-Mouse CD8a PE-Cyanine5 488 - 561 nm 667 nm FC RUO
86-0081 Anti-Mouse CD8a eVolve™ 655 FC RUO
61-0081 Anti-Mouse CD8a PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
42-0081 Anti-Mouse CD8a eFluor® 615 595 nm 615 nm ICC, IHC, IHC-F RUO
69-0081 Anti-Mouse CD8a eFluor® 506 405 nm 506 nm FC RUO
16-0081 Anti-Mouse CD8a Functional Grade Purified FC, FA RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
17-0032 Anti-Mouse CD3 APC 633 - 647 nm 660 nm FC RUO
SB-4400 Super Bright Staining Buffer FC GPR
63-4321 Rat IgG2a K Isotype Control Super Bright 600 405 nm 600 nm FC RUO
References

References: Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (53-6.7, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (53-6.7, in vivo depletion, PubMed)

Taylor JL, Ordway DJ, Troudt J, Gonzalez-Juarrero M, Basaraba RJ, Orme IM. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun. 2005 Aug;73(8):5189-93. (53-6.7, IHC frozen)

Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K. Beta2 integrins are required for skin homing of primed T cells but not for priming naïve T cells. J Clin Invest. 2002 Jan;109(2):183-92. (53-6.7, IHC frozen)

Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95.

Ledbetter, J. A. and L. A. Herzenberg. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90.


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