Anti-Mouse CD86 (B7-2) eFluor® 605NC

Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 & CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

References: Hathcock, K. S., G. Laszlo, et al. (1994). Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J Exp Med 180(2): 631-40

Inaba, K., M. Witmer-Pack, et al. (1994). The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180(5): 1849-60.

Hathcock, K. S., G. Laszlo, et al. (1993). Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science 262(5135): 905-7.

Freeman, G. J., F. Borriello, et al. (1993). Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production. J Exp Med 178(6): 2185-92.