Anti-Mouse CD79a eFluor® 660 (Alexa Fluor® 647 Replacement)

Description: This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a (also known as Ig alpha or mb-1), a signaling component of the B cell receptor (BCR). CD79a heterodimerizes with CD79b (Ig beta, B29), and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain. CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias. The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs (ITAMs), which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.

References: Patterson HC, Kraus M, Kim YM, Ploegh H, Rajewsky K. The B cell receptor promotes B cell activation and proliferation through a non-ITAM tyrosine in the Igalpha cytoplasmic domain. Immunity. 2006 Jul;25(1):55-65.

Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ. The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172(4):2210-8.

Melenz D, Ruschel A, Vettermann C, Jäck HM. Immunoglobulin mu heavy chains do not mediate tyrosine phosphorylation of Ig alpha from the ER-cis-Golgi. J Immunol. 2003 ;171(6):3091-101. (24C2.5, FC, WB, Pubmed)

Astsaturov IA, Matutes E, Morilla R, Seon BK, Mason DY, Farahat N, Catovsky D. Differential expression of B29 (CD79b) and mb-1 (CD79a) proteins in acute lymphoblastic leukaemia. Leukemia. 1996 May;10(5):769-73.