Anti-Mouse CD47 APC

Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with ß3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

No crossreactivity is seen against human CD47.

References: Chang HP, Ma YL, Wan FJ, Tsai LY, Lindberg FP, Lee EH. Functional blocking of integrin-associated protein impairs memory retention and decreases glutamate release from the hippocampus. Neuroscience. 2001;102(2):289-96

Babic I, Schallhorn A, Lindberg FP, Jirik FR. SHPS-1 induces aggregation of Ba/F3 pro-B cells via an interaction with CD47. J Immunol. 2000 Apr 1;164(7):3652-8. (miap301, FA (blocking cell aggregation in vitro), PubMed)

Jiang P, Lagenaur CF, Narayanan V. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. J Biol Chem. 1999 Jan 8;274(2):559-62.

Mouro-Chanteloup I, Delaunay J, et al. 2003. Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47. Blood 101(1):338-44. (miap301, WB, PubMed)

Chang HP, Lindberg FP, et al. 1999. Impaired memory retention and decreased long-term potentiation in integrin-associated protein-deficient mice. (miap301, WB, PubMed)