Anti-Mouse CD4 Purified

Also known as: L3T4, Ly-4

Clone: RM4-5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 14-0042

Cat. No. Size
14-0042-81 50 ug
14-0042-82 100 ug
14-0042-85 500 ug
14-0042-86 1 mg

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Data for Anti-Mouse CD4 Purified.

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  • Data for Anti-Mouse CD4 Purified.
Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Mouse
Conjugate Purified
Reported Applications Flow Cytometric Analysis, Immunohistochemical Staining of Frozen Tissue Sections, Functional Assays
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
15-0042 Anti-Mouse CD4 PE-Cy5 488 nm 670 nm FC RUO
25-0042 Anti-Mouse CD4 PE-Cy7 488 nm 760 nm FC RUO
42-0042 Anti-Mouse CD4 eFluor® 615 ICC, IHC RUO
IH95-0042 Anti-Mouse CD4 eFluor® 650NC (for IHC/ICC) 350 - 635 nm 650 nm ICC, IHC RUO
12-0042 Anti-Mouse CD4 PE 488 nm 575 nm FC RUO
48-0042 Anti-Mouse CD4 eFluor® 450 405 nm 450 nm FC RUO
46-0042 Anti-Mouse CD4 PerCP-eFluor® 710 488 nm 710 nm FC RUO
17-0042 Anti-Mouse CD4 APC 633 nm 660 nm FC RUO
IH93-0042 Anti-Mouse CD4 eFluor® 605NC (for IHC/ICC) 350 - 590 nm 605 nm ICC, IHC RUO
IH94-0042 Anti-Mouse CD4 eFluor® 625NC (for IHC/ICC) 350 - 610 nm 625 nm ICC, IHC RUO
56-0042 Anti-Mouse CD4 Alexa Fluor® 700 633 nm 723 nm FC RUO
47-0042 Anti-Mouse CD4 APC-eFluor® 780 633 nm 780 nm FC RUO
11-0042 Anti-Mouse CD4 FITC 488 nm 518 nm FC RUO
13-0042 Anti-Mouse CD4 Biotin FC RUO
45-0042 Anti-Mouse CD4 PerCP-Cy5.5 488 nm 690 nm FC RUO
16-0042 Anti-Mouse CD4 Functional Grade Purified FC RUO
References

References: Bliss SK, Bliss SP, Beiting DP, Alcaraz A, Appleton JA. IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007 Jun 15;178(12):7974-83. (RM4-5, FC, PubMed)

Rubinstein MP, Kovar M, Purton JF, Cho JH, Boyman O, Surh CD, Sprent J. Converting IL-15 to a superagonist by binding to soluble IL-15R alpha. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9166-71. (RM4-5), FC, PubMed)

Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, Ridgway WM. NOD.c3c4 congenic mice develop autoimmune biliary disease that serologically and pathogenetically models human primary biliary cirrhosis. J Exp Med. 2006 May 15;203(5):1209-19. (RM4-5, IHC frozen, PubMed)

Andres PG, Beck PL, Mizoguchi E, Mizoguchi A, Bhan AK, Dawson T, Kuziel WA, Maeda N, MacDermott RP, Podolsky DK, Reinecker HC. Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis: lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine. J Immunol. 2000 Jun 15;164(12):6303-12. (RM4-5, IHC frozen)

Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, Datta SK. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998 Feb 2;187(3):367-78. (RM4-5, FA)

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.


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