Anti-Mouse CD4 Alexa Fluor® 647 (To Be Discontinued. Refer to Replacement Format eFluor® 660, cat. 50-0041)

Also known as: L3T4, Ly-4

Clone: GK1.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: To Be Discontinued. Refer to Replacement Format® eFluor 660, cat. 50-0041

SKU# 51-0041

Cat. No.	Size
51-0041-80	25 ug
51-0041-82	100 ug

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Description

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
53-0041	Anti-Mouse CD4 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
14-0041	Anti-Mouse CD4 Purified			FC, IHC, IP	RUO
25-0041	Anti-Mouse CD4 PE-Cy7	488 nm	760 nm	FC	RUO
95-0041	Anti-Mouse CD4 eFluor® 650NC	350 - 635 nm	650 nm	FC	RUO
16-0041	Anti-Mouse CD4 Functional Grade Purified			FC, FA	RUO
36-0041	Anti-Mouse CD4 Functional Grade Biotin			FC, IHC, IP	RUO
93-0041	Anti-Mouse CD4 eFluor® 605NC	350 - 590 nm	605 nm	FC	RUO
17-0041	Anti-Mouse CD4 APC	633 nm	660 nm	FC	RUO
19-0041	Anti-Mouse CD4 Cy5	633 nm	670 nm	FC	RUO
11-0041	Anti-Mouse CD4 FITC	488 nm	518 nm	FC	RUO
12-0041	Anti-Mouse CD4 PE	488 nm	575 nm	FC	RUO
50-0041	Anti-Mouse CD4 eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
13-0041	Anti-Mouse CD4 Biotin			FC	RUO
48-0041	Anti-Mouse CD4 eFluor® 450	405 nm	450 nm	FC	RUO
56-0041	Anti-Mouse CD4 Alexa Fluor® 700	633 nm	723 nm	FC	RUO
15-0041	Anti-Mouse CD4 PE-Cy5	488 nm	670 nm	FC	RUO

References

References: Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed)

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.

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Antigens	Area of Biology	Cell Type	Formats
CD4	Adaptive Immunity	Macrophage & Monocyte Cells T Cells Th17 Cells Treg Cells	Alexa Fluor 647