Anti-Mouse CD3e Alexa Fluor® 488

Also known as: CD3 epsilon

Clone: 145-2C11

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0031

Cat. No.	Size
53-0031-80	25 ug
53-0031-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg...View More

Description

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Details

Host	Armenian Hamster
Isotype	IgG
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-0031	Anti-Mouse CD3e eFluor® 450	405 nm	450 nm	FC	RUO
17-0031	Anti-Mouse CD3e APC	633 - 647 nm	660 nm	FC	RUO
11-0031	Anti-Mouse CD3e FITC	488 nm		FC	RUO
25-0031	Anti-Mouse CD3e PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
12-0031	Anti-Mouse CD3e PE	488 - 561 nm	575 nm	FC	RUO
14-0031	Anti-Mouse CD3e Purified			FC, IHC, FA, IP, WB	RUO
13-0031	Anti-Mouse CD3e Biotin			FC	RUO
16-0031	Anti-Mouse CD3e Functional Grade Purified			FC, FA	RUO
35-0031	Anti-Mouse CD3e PE-Cyanine5.5	488 - 567 nm	690 nm	FC	RUO
36-0031	Anti-Mouse CD3e Functional Grade Biotin			FC, IHC, FA, IP, WB	RUO
47-0031	Anti-Mouse CD3e APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO
15-0031	Anti-Mouse CD3e PE-Cyanine5	488 - 561 nm		FC	RUO
45-0031	Anti-Mouse CD3e PerCP-Cyanine5.5	488 nm		FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-4888	Armenian Hamster IgG Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Geng H, Zhang GM, Xiao H, Yuan Y, Li D, Zhang H, Qiu H, He YF, Feng ZH. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer. 2006 Jun 1;118(11):2657-64. (145-2C11, IHC frozen, PubMed)

Krieg C, Han P, Stone R, Goularte OD, Kaye J. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol. 2005 Nov 15;175(10):6420-7. (145-2C11, FA, PubMed)

Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M. CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response. Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3753-60. (145-2C11, IHC frozen, PubMed)

Schuchert MJ, Wright RD, Colson YL. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (145-2C11, WB and IP)

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (145-2C11, IP and WB)

Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14.

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8.

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Antigens	Area of Biology	Cell Type	Formats
CD3e	Adaptive Immunity	NK & NKT Cells T Cells Th17 Cells Treg Cells	Alexa Fluor 488