Anti-Mouse CD39 eFluor® 660 (Alexa® 647 Replacement)

Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.

References: Hyman MC, Petrovic-Djergovic D, Visovatti SH, Liao H, Yanamadala S, Bouïs D, Su EJ, Lawrence DA, Broekman MJ, Marcus AJ, Pinsky DJ. Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39. J Clin Invest. 2009 May;119(5):1136-49. (24DMS1, FC, PubMed)

Bynoe MS, Viret C. Foxp3+CD4+ T cell-mediated immunosuppression involves extracellular nucleotide catabolism.Trends Immunol. 2008 Mar;29(3):99-102.

Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, Höpner S, Centonze D, Bernardi G, Dell'Acqua ML, Rossini PM, Battistini L, Rötzschke O, Falk K. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007 Aug 15;110(4):1225-32.

Deaglio S, Dwyer KM, Gao W, Friedman D, Usheva A, Erat A, Chen JF, Enjyoji K, Linden J, Oukka M, Kuchroo VK, Strom TB, Robson SC. Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med. 2007 Jun 11;204(6):1257-65.

Mizumoto N, Kumamoto T, Robson SC, Sévigny J, Matsue H, Enjyoji K, Takashima A. CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness.Nat Med. 2002 Apr;8(4):358-65.

Sévigny J, Sundberg C, Braun N, Guckelberger O, Csizmadia E, Qawi I, Imai M, Zimmermann H, Robson SC.Differential catalytic properties and vascular topography of murine nucleoside triphosphate diphosphohydrolase 1 (NTPDase1) and NTPDase2 have implications for thromboregulation.Blood. 2002 Apr 15;99(8):2801-9.

Braun N, Sévigny J, Robson SC, Enjyoji K, Guckelberger O, Hammer K, Di Virgilio F, Zimmermann H.Assignment of ecto-nucleoside triphosphate diphosphohydrolase-1/cd39 expression to microglia and vasculature of the brain.Eur J Neurosci. 2000 Dec;12(12):4357-66.

Enjyoji K, Sévigny J, Lin Y, Frenette PS, Christie PD, Esch JS 2nd, Imai M, Edelberg JM, Rayburn H, Lech M, Beeler DL, Csizmadia E, Wagner DD, Robson SC, Rosenberg RD.Targeted disruption of cd39/ATP diphosphohydrolase results in disordered hemostasis and thromboregulation.Nat Med. 1999 Sep;5(9):1010-7.