Anti-Mouse CD335 (NKp46) Alexa Fluor® 647 (To Be Discontinued. Refer to Replacement Format eFluor® 660, cat. 50-3351)

Also known as: NCR1, Ly-94, Ly94

Clone: 29A1.4

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: To Be Discontinued. Refer to Replacement Format eFluor® 660 cat. 50-3351

SKU# 51-3351

Cat. No.	Size
51-3351-80	25 ug
51-3351-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Description

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
16-3351	Anti-Mouse CD335 (NKp46) Functional Grade Purified			FC, FA	RUO
12-3351	Anti-Mouse CD335 (NKp46) PE	488 - 561 nm	575 nm	FC	RUO
11-3351	Anti-Mouse CD335 (NKp46) FITC	488 nm	518 nm	FC	RUO
50-3351	Anti-Mouse CD335 (NKp46) eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
48-3351	Anti-Mouse CD335 (NKp46) eFluor® 450	405 nm	450 nm	FC	RUO
46-3351	Anti-Mouse CD335 (NKp46) PerCP-eFluor® 710	488 nm	710 nm	FC	RUO

References

References: Walzer T, Chiossone L, Chaix J, Calver A, Carozzo C, Garrigue-Antar L, Jacques Y, Baratin M, Tomasello E, Vivier E. Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor.Nat Immunol. 2007 Dec;8(12):1337-44. (29A1.4, FC PubMed)

Walzer T, Bléry M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, André P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagné F, Vivier E. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46.Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3384-9. (29A1.4, FC, FA PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD335 (NKp46)	Innate Immunity	NK & NKT Cells	Alexa Fluor 647