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Anti-Mouse CD3 Alexa Fluor® 532

Clone: 17A2

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 58-0032

Cat. No. Size
58-0032-80 25 ug
58-0032-82 100 ug

Data for Anti-Mouse CD3 Alexa Fluor® 532.

Staining of BALB/c splenocytes with Anti-Mouse CD19 APC (cat. 17-0193) and autofluoresence (left) or...View More

  • Data for Anti-Mouse CD3 Alexa Fluor® 532.

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Host Rat
Isotype IgG2b, kappa
Reactivity Mouse
Conjugate Alexa Fluor 532
Laser Green Laser
Emit 561 nm
Excite 532 nm
Legal Alexa Fluor® is a trademark of Life Technologies Corporation.
Reported Applications Flow Cytometric Analysis
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
46-0032 Anti-Mouse CD3 PerCP-eFluor® 710 488 nm 710 nm FC RUO
69-0032 Anti-Mouse CD3 eFluor® 506 405 nm 506 nm FC RUO
11-0032 Anti-Mouse CD3 FITC 488 nm 520 nm FC, IHC, IHC-F RUO
47-0032 Anti-Mouse CD3 APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
17-0032 Anti-Mouse CD3 APC 633 - 647 nm 660 nm FC RUO
50-0032 Anti-Mouse CD3 eFluor® 660 633 - 647 nm 668 nm FC, IHC, IHC-F RUO
16-0032 Anti-Mouse CD3 Functional Grade Purified FC, FA RUO
48-0032 Anti-Mouse CD3 eFluor® 450 405 nm 445 nm FC RUO
13-0032 Anti-Mouse CD3 Biotin FC RUO
56-0032 Anti-Mouse CD3 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
14-0032 Anti-Mouse CD3 Purified FC, IHC, IHC-F, FA RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
17-0193 Anti-Mouse CD19 APC 633 - 647 nm 660 nm FC RUO
00-4300 10X RBC Lysis Buffer (Multi-species) FC, FA GPR
58-4031 Rat IgG2b K Isotype Control Alexa Fluor® 532 532 nm 561 nm FC RUO

References: Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F, PubMed)

Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.