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Anti-Mouse CD274 (PD-L1, B7-H1) Super Bright 436

Clone: MIH5

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 62-5982

Cat. No. Size
62-5982-80 25 ug
62-5982-82 100 ug

Data for Anti-Mouse CD274 (PD-L1, B7-H1) Super Bright 436.

Staining of BALB/c splenocytes with Anti-Mouse CD3e APC (cat. 17-0031) and 0.125 ug of Rat IgG2a K...View More

  • Data for Anti-Mouse CD274 (PD-L1, B7-H1) Super Bright 436.
Description

Description: The MIH5 monoclonal antibody reacts with mouse CD274, also known as B7-H1 or PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Details
Host Rat
Isotype IgG2a, lambda
Reactivity Mouse
Conjugate Super Bright 436
Laser Violet Laser
Emit 436 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
25-5982 Anti-Mouse CD274 (PD-L1, B7-H1) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
16-5982 Anti-Mouse CD274 (PD-L1, B7-H1) Functional Grade Purified FC, FA RUO
13-5982 Anti-Mouse CD274 (PD-L1, B7-H1) Biotin FC RUO
46-5982 Anti-Mouse CD274 (PD-L1, B7-H1) PerCP-eFluor® 710 488 nm 710 nm FC RUO
12-5982 Anti-Mouse CD274 (PD-L1, B7-H1) PE 488 - 561 nm 578 nm FC RUO
14-5982 Anti-Mouse CD274 (PD-L1, B7-H1) Purified FC, IHC, FA, WB RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
17-0031 Anti-Mouse CD3e APC 633 - 647 nm 660 nm FC RUO
SB-4400 Super Bright Staining Buffer FC GPR
62-4321 Rat IgG2a K Isotype Control Super Bright 436 405 nm 436 nm FC RUO
References

References: Sheng H, Wang Y, Jin Y, Zhang Q, Zhang Y, Wang L, Shen B, Yin S, Liu W, Cui L, Li N. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res. 2008 Aug;18(8):846-57. (MIH5, FC, PubMed)

Saudemont A, Jouy N, Hetuin D, Quesnel B. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 2005 Mar 15;105(6):2428-35. (MIH5, FA, PubMed)

Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, W├╝thrich RP. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004 Nov;19(11):2713-20. (MIH5, FA, WB, IHC frozen, PubMed)

Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004 Jan 15;77(1):6-12.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63.

Tsushima F, Iwai H, Otsuki N, Abe M, Hirose S, Yamazaki T, Akiba H, Yagita H, Takahashi Y, Omura K, Okumura K, Azuma M. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses. Eur J Immunol. 2003 Oct;33(10):2773-82. (MIH5, FA, IHC frozen)

Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ, Malenkovich N, Okazaki T, Byrne MC, Horton HF, Fouser L, Carter L, Ling V, Bowman MR, Carreno BM, Collins M, Wood CR, Honjo T. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34.

Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999 Dec;5(12):1365-9.