Anti-Mouse CD25 Purified

Also known as: Interleukin-2 Receptor alpha

Clone: PC61.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 14-0251

Cat. No. Size
14-0251-81 50 ug
14-0251-85 500 ug
14-0251-86 1 mg
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Data for Anti-Mouse CD25 Purified.

Staining of 3-day ConA activated C57Bl/6 splenocytes with 0.25 ug of Rat IgG1 Isotype Control...View More

  • Data for Anti-Mouse CD25 Purified.
Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details
Host Rat
Isotype IgG1, lambda
Reactivity Mouse
Conjugate Purified
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
35-0251 Anti-Mouse CD25 PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
45-0251 Anti-Mouse CD25 PerCP-Cyanine5.5 488 nm FC RUO
48-0251 Anti-Mouse CD25 eFluor® 450 405 nm 450 nm FC RUO
16-0251 Anti-Mouse CD25 Functional Grade Purified FC, FA RUO
13-0251 Anti-Mouse CD25 Biotin FC RUO
56-0251 Anti-Mouse CD25 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
15-0251 Anti-Mouse CD25 PE-Cyanine5 488 - 561 nm FC RUO
12-0251 Anti-Mouse CD25 PE 488 - 561 nm 575 nm FC RUO
53-0251 Anti-Mouse CD25 Alexa Fluor® 488 488 nm 519 nm FC RUO
47-0251 Anti-Mouse CD25 APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
36-0251 Anti-Mouse CD25 Functional Grade Biotin FC RUO
25-0251 Anti-Mouse CD25 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
17-0251 Anti-Mouse CD25 APC 633 - 647 nm 660 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-4317 Streptavidin FITC 488 nm FC, ICC, IHC RUO
11-4811 Anti-Rat IgG FITC 488 nm FC, ICC, IHC RUO
12-4317 Streptavidin PE 488 - 561 nm FC RUO
13-4813 Anti-Rat IgG Biotin FC, ELISA, IHC, WB RUO
14-4301 Rat IgG1 K Isotype Control Purified FC, ICC, IHC RUO
17-4317 Streptavidin APC 633 - 647 nm 660 nm FC RUO
References

References: Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.